概述

  • 产品名称
    人Angiogenin ELISA试剂盒(ANG)
    参阅全部 Angiogenin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall < 10%
    批次间
    样品 n Mean SD CV%
    Overall < 12%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 1.5 pg/ml
  • 范围
    1.64 pg/ml - 400 pg/ml
  • 复检

    94.7 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.37 85% - 104%
    Serum 95.95 82% - 105%
    Plasma 93.49 83% - 104%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Angiogenin (ANG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiogenin in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human Angiogenin coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiogenin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiogenin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiogenin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab99970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab99970.

  • Representative standard curve using ab99970.

实验方案

文献

This product has been referenced in:
  • Ting KR  et al. Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients. BBA Clin 8:28-34 (2017). Read more (PubMed: 28725572) »
  • Miyake M  et al. Angiogenin promotes tumoral growth and angiogenesis by regulating matrix metallopeptidase-2 expression via the ERK1/2 pathway. Oncogene N/A:N/A (2014). Read more (PubMed: 24561529) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for contacting us. I have confirmed that Assay Diluent B would be the best diluent to use if testing tears.  Assay Diluent A is specifically optimized to simulate the serum/plasma matrix while Assay Diluent B is better suited for a variety of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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