人Angiogenesis抗体阵列(10 Targets)- Quantitative (ab197419)
概述
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产品名称
人Angiogenesis抗体阵列(10 Targets)- Quantitative
参阅全部 Angiogenesis Antibody Array 抗体芯片 -
样品类型
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
检测类型
Quantitative -
检测时间
6h 00m -
种属反应性
与反应: Human -
产品概述
ab197419 can be used for quantitative measurement of 10 Human Angiogenesis proteins. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Targets names: Angiogenin, Angiopoietin-2, EGF, bFGF, HB-EGF, HGF, Leptin, PDGF-BB, PIGF and VEGF-A.
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说明
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.
The standard is affinity purified.
Learn more about quantitative antibody arrays
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 50 tests 22 tests 8 tests 8 tests 20X Wash Buffer I 6 x 30ml 3 x 30ml 2 x 30ml 2 x 30ml 20X Wash Buffer II 2 x 30ml 1 x 30ml 1 x 30ml 1 x 30ml Cy3 equivalent dye-conjugated Streptavidin 4 x 5µl 2 x 5µl 1 x 5µl 1 x 5µl Human Angiogenesis Array (10 T) Biotinylated Antibody Cocktail 4 units 2 units 1 unit 1 unit Human Angiogenesis Array (10 T) Glass Slide 4 slides 2 slides 1 slide 1 slide Human Angiogenesis Array (10 T) Lyophilized Cytokine Standard Mix 2 vials 1 vial 1 vial 1 vial Sample Diluent 2 x 15ml 1 x 15ml 1 x 15ml 1 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 2 units 1 unit 1 unit 1 unit -
研究领域
图片
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Assay summary for Abcam Human Angiogenesis Antibody Array (10 Targets) - Quantitative (ab197419).
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Array map for Abcam Human Angiogenesis Antibody Array (10 Targets) - Quantitative (ab197419).
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Standard Curve obtained using Abcam Human Angiogenesis Antibody Array (10 Targets) - Quantitative (ab197419).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab197419 被引用在 1 文献中.
- Qazi TH et al. Dosage and composition of bioactive glasses differentially regulate angiogenic and osteogenic response of human MSCs. J Biomed Mater Res A 106:2827-2837 (2018). PubMed: 30281904