人Amphiregulin ELISA试剂盒(AREG) (ab99975)

概述

  • 产品名称人Amphiregulin ELISA试剂盒(AREG)
    参阅全部 Amphiregulin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    16.46 pg/ml - 4000 pg/ml
  • 复检

    83 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 100.4 89% - 111%
    Serum 85 74% - 95%
    Plasma 72.7 64% - 87%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Amphiregulin (AREG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in serum (Human Amphiregulin concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性The mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
  • 细胞定位Cell Membrane
  • 别名
    • A REG
    • AR
    • AREG
    • Colorectum cell derived growth factor
    • CRDGF
    • MGC13647
    • OTTHUMP00000219184
    • Schwannoma derived growth factor
    • SDGF
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Amphiregulin ELISA Kit (AREG) 图像

  • Representative Standard Curve using ab99975.

  • Representative Standard Curve using ab99975.

实验方案

Human Amphiregulin ELISA Kit (AREG) (ab99975)参考文献

ab99975 has not yet been referenced specifically in any publications.

Product Wall



A lot of our kits are very accommodating to many different samples types so lysates will likely work. If you decide to tests this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay Diluent B to minimize any...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"