人alpha Fetoprotein ELISA试剂盒(AFP) (ab108838)

概述

  • 产品名称人alpha Fetoprotein ELISA试剂盒(AFP)
    参阅全部 alpha 1 Fetoprotein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.15 ng/ml
  • 范围
    0.156 ng/ml - 10 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 0m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum and cell culture supernatants.

    An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Afp
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human alpha Fetoprotein ELISA Kit (AFP) 图像

  • Representative Standard Curve using ab108838.

实验方案

Human alpha Fetoprotein ELISA Kit (AFP) (ab108838)参考文献

ab108838 has not yet been referenced specifically in any publications.

Product Wall

The solubility is very good. It can go to higher than 10 ug/ml. If you do in the ng/ml range, it will be in the >500 ng/ml

Let me know if there is anything else I can help you with.

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The alpha Fetoprotein Human ELISA Kit (ab108838) has not had FDA approval but this is not necessary if the kit is to be used for research purposes only. All of our products are not intended to be used for diagnost...

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Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"