人alpha Fetoprotein ELISA试剂盒(ab108680)

概述

  • 产品名称人alpha Fetoprotein ELISA试剂盒
    参阅全部 alpha 1 Fetoprotein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Control Sera 48 <=6.4%
    批次间
    样品 n Mean SD CV%
    Control Sera 48 <=6.5%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.35 ng/ml
  • 范围
    5 ng/ml - 200 ng/ml
  • 复检

    98.33 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha Fetoprotein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in serum and plasma.

     

    A 96-well plate has been precoated with anti-alpha Fetoprotein IgG antibodies. Samples, standards and the alpha Fetoprotein-HRP conjugate are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody and added alpha Fetoprotein-HRP conjugate binds to this antibody-AFP complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Afp
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab108680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human alpha Fetoprotein ELISA Kit 图像

  • Representative Standard Curve using ab108680

实验方案

Human alpha Fetoprotein ELISA Kit (ab108680)参考文献

ab108680 has not yet been referenced specifically in any publications.

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