人alpha Fetoprotein ELISA试剂盒(ab108631)

概述

  • 产品名称
    人alpha Fetoprotein ELISA试剂盒
    参阅全部 alpha 1 Fetoprotein 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 2 ng/ml
  • 范围
    2 ng/ml - 300 ng/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha Fetoprotein (AFP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of alpha Fetoprotein in Human serum.

    A 96-well plate has been precoated with anti-alpha Fetoprotein antibodies. Samples and standards and are added to the wells, where alpha Fetoprotein in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-alpha Fetoprotein-HRP conjugate binds to the antibody-alpha Fetoprotein complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of alpha Fetoprotein in the sample and the intensity is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    alpha Fetoprotein Standard - 0 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 150 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 20 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 300 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 5 ng/mL 1 x 0.5ml
    alpha Fetoprotein Standard - 50 ng/mL 1 x 0.5ml
    Anti-alpha Fetoprotein Coated Microplate (12 x 8 wells) 1 unit
    Anti-alpha Fetoprotein HRP Conjugate 1 x 18ml
    Stop Solution 1 x 11ml
    TMB Substrate Solution 1 x 11ml
    Zero Buffer 1 x 13ml
  • 研究领域
  • 功能
    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
  • 组织特异性
    Plasma. Synthesized by the fetal liver and yolk sac.
  • 序列相似性
    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 发展阶段
    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.
  • 翻译后修饰
    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Afp
    • AFPD
    • Alpha fetoglobulin
    • Alpha fetoprotein
    • Alpha fetoprotein precursor
    • Alpha-1-fetoprotein
    • Alpha-fetoglobulin
    • Alpha-fetoprotein
    • alpha-fetoprotein, Hereditary persistence of, included
    • FETA
    • FETA_HUMAN
    • Hereditary persistence of alpha fetoprotein
    • HPAFP
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

图片

  • Representative standard curve using ab108631

实验方案

文献

ab108631 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. I can confirm that all the mentioned kits (ab99992, ab108631, ab108635, ab108838) come with a 96-well strip plate (12x8).  Each strip (12 total) has 8 wells so the customer can use a strip at a time if they wish. I ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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