人alpha 2 Macroglobulin ELISA试剂盒(ab108888)

概述

  • 产品名称人alpha 2 Macroglobulin ELISA试剂盒
    参阅全部 alpha 2 Macroglobulin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Cell Lysate
  • 检测类型Competitive
  • 灵敏度
    = 1 µg/ml
  • 范围
    1.25 µg/ml - 40 µg/ml
  • 复检

    96 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.

    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Human alpha 2 Macroglobulin ELISA Kit 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 2 Macroglobulin measured in biological fluids and cell culture supernatant showing quantity (ug) per mL of tested sample. Human serum diluted 400-1600 fold. Human plasma diluted 80-320 fold. Other samples undiluted.

实验方案

Human alpha 2 Macroglobulin ELISA Kit (ab108888)参考文献

This product has been referenced in:
  • Sunderic M  et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). Read more (PubMed: 25106097) »

See 1 Publication for this product

Product Wall

The lab has informed me that your sample collection method is fine and that the anti-coagulant will not affect the assay. You can include the anticoagulant.

Thank you for contacting us.

The lab said the other option besides sodium citrate 0.1M is the ready use tube that clinical lab used to draw
human plasma for your samples.


I hope this information is helpful to you. Pleas...

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Thank you for contacting us.

Unfortunately the laboratory has no data regarding on ATC samples, and cannot give a recommendation here.

Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your call yesterday and for your patience while I've been in touch with my colleagues regarding this standard reconstitution volume.

For this lot, the standard vial contains 160 ug of human alpha-2-macroglobulin. So, t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"