人alpha 2 Macroglobulin ELISA试剂盒(ab108888)
Key features and details
- Sensitivity: 1.1 µg/ml
- Range: 1.25 µg/ml - 40 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
概述
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产品名称
人alpha 2 Macroglobulin ELISA试剂盒
参阅全部 alpha 2 Macroglobulin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5.9% 批次间 样品 n Mean SD CV% Overall 10% -
样品类型
Serum, Plasma -
检测类型
Competitive -
灵敏度
= 1.1 µg/ml -
范围
1.25 µg/ml - 40 µg/ml -
回收率
97 %
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检测时间
3h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s alpha 2 Macroglobulin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in plasma and serum.
An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 2 Macroglobulin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 2 Macroglobulin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml 1X Biotinylated alpha 2 Macroglobulin (Lyophilized) 1 vial alpha 2 Macroglobulin Microplate (12 x 8 well strips) 1 unit alpha 2 Macroglobulin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
组织特异性
Secreted in plasma. -
序列相似性
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
发展阶段
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
细胞定位
Secreted. - Information by UniProt
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别名
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
数据库链接
- Entrez Gene: 2 Human
- Omim: 103950 Human
- SwissProt: P01023 Human
- Unigene: 212838 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab108888 被引用在 7 文献中.
- Byrne JF et al. Transdiagnostic inflammatory subgroups among psychiatric disorders and their relevance to role functioning: a nested case-control study of the ALSPAC cohort. Transl Psychiatry 12:377 (2022). PubMed: 36085284
- Gupta AK et al. Cerebrospinal Fluid Proteomics For Identification Of a2-Macroglobulin As A Potential Biomarker To Monitor Pharmacological Therapeutic Efficacy In Dopamine Dictated Disease States Of Parkinson's Disease And Schizophrenia. Neuropsychiatr Dis Treat 15:2853-2867 (2019). PubMed: 31632033
- Ghale-Noie ZN et al. High Serum Alpha-2-Macroglobulin Level in Patients with Osteonecrosis of the Femoral Head. Arch Bone Jt Surg 6:219-224 (2018). PubMed: 29911139
- Bjelosevic S et al. Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults. Mol Cell Proteomics 16:924-935 (2017). PubMed: 28336724
- Robinson MD et al. Water T2as an early, global and practical biomarker for metabolic syndrome: an observational cross-sectional study. J Transl Med 15:258 (2017). PubMed: 29258604
- Chen X et al. A proteomics analysis reveals that A2M might be regulated by STAT3 in persistent allergic rhinitis. Clin Exp Allergy 46:813-24 (2016). PubMed: 27228572
- Sunderic M et al. Protein molecular forms of insulin-like growth factor binding protein-2 change with aging. Exp Gerontol 58C:154-158 (2014). PubMed: 25106097