人alpha 2 Macroglobulin ELISA试剂盒(ab108883)

概述

  • 产品名称人alpha 2 Macroglobulin ELISA试剂盒
    参阅全部 alpha 2 Macroglobulin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.4%
    批次间
    样品 n Mean SD CV%
    Overall 8.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 2 ng/ml
  • 范围
    0.002 µg/ml - 0.5 µg/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha 2 Macroglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 2 Macroglobulin in saliva, milk, cerebrospinal fluid and cell culture supernatants.

    An alpha 2 Macroglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 2 Macroglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 2 Macroglobulin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 组织特异性Secreted in plasma.
  • 序列相似性Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • 发展阶段Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human alpha 2 Macroglobulin ELISA Kit 图像

  • Representative Standard Curve using ab108883.

Human alpha 2 Macroglobulin ELISA Kit (ab108883)参考文献

This product has been referenced in:
  • Sturtzel C  et al. The transcription factor MEF2C negatively controls angiogenic sprouting of endothelial cells depending on oxygen. PLoS One 9:e101521 (2014). Read more (PubMed: 24988463) »

See 1 Publication for this product

Product Wall



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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Abreview for alpha 2 Macroglobulin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.125ug standard µg
Type of Kit Used: ELISA
Positive control: Provided alpha 2 Macroglobulin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human A2M. Did not recognize bovine A2M in FBS.
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Abcam user community

Verified customer

提交于 Jul 03 2012

Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfami...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"