人alpha 1 Microglobulin ELISA试剂盒(ab108884)

概述

  • 产品名称人alpha 1 Microglobulin ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.3%
    批次间
    样品 n Mean SD CV%
    Overall 8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.2 ng/ml
  • 范围
    0.313 ng/ml - 20 ng/ml
  • 复检

    96 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha 1 Microglobulin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Microglobulin concentrations in Human cell culture supernatants, serum, plasma, urine, saliva, CSF and milk.

    An alpha 1 Microglobulin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 Microglobulin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 Microglobulin captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • 组织特异性Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • 序列相似性In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • 翻译后修饰The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    N- and O-glycosylated. N-glycan heterogeneity at Asn-115: Hex5HexNAc4 (major), Hex6HexNAc5 (minor) and dHex1Hex6HexNAc5 (minor). N-glycan at Asn-250: Hex5HexNAc4. O-linkage of the glycosaminoglycan, chondroitin sulfate, at Ser-215 allows cross-linking between the three polypeptide chains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Alpha-1 microglycoprotein
    • AMBP
    • AMBP_HUMAN
    • Bikunin
    • Complex-forming glycoprotein heterogeneous in charge
    • EDC1
    • HI-30
    • ITI-LC
    • Protein HC
    • Trypstatin
    • Uronic-acid-rich protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human alpha 1 Microglobulin ELISA Kit 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Microglobulin measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 10000-40000 fold. Human urine and milk diluted 100-800 fold. Human saliva diluted 8-16 fold. HepG2 supernatant diluted 1000 fold. MG-63 supernatant tested undiluted - below detection. Mouse and rat samples diluted 10 fold - below detection.

实验方案

Human alpha 1 Microglobulin ELISA Kit (ab108884)参考文献

ab108884 has not yet been referenced specifically in any publications.

Product Wall



Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provi...

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Thank you for your inquiry. ab108884 is an alpha 1 microglobulin human ELISA kit, Swiss Prot P02760. Alpha1-microglobulin (1M), also called protein HC, is a tubular plasma and tissue protein that belongs to the lipocalin transport protein superfami...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"