人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1) (ab108799)

概述

  • 产品名称人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1)
    参阅全部 alpha 1 Antitrypsin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Cell culture media, Lithium Heparin Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.35 ng/ml
  • 范围
    0.391 ng/ml - 100 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha 1 Antitrypsin (SERPINA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 Antitrypsin in urine, milk, saliva, CSF and cell culture supernatants.


    An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha 1 antitrypsin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha 1 antitrypsin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 组织特异性Ubiquitous. Expressed in leukocytes and plasma.
  • 疾病相关Alpha-1-antitrypsin deficiency
  • 序列相似性Belongs to the serpin family.
  • 结构域The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻译后修饰N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 细胞定位Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 别名
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108799 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) 图像

  • Linearity: alpha 1 antitrypsin in urine at different diutions, corrected for the factor of dilution (duplicates +/- SD).

  • Linearity: alpha 1 antitrypsin in human milk at different diutions, corrected for the factor of dilution (duplicates +/- SD)

  • Linearity: alpha 1 antitrypsin in serum and plasma (Li-heparine) at different diutions, corrected for the factor of dilution (duplicates +/- SD)

  • Alpha 1 antitrypsin in human biological fluids (duplicates +/- SD).

    The samples were tested in different ranges of dilutions (serum and Li-heparine plasma: 2e3-1e6; CSF: 5e3-5e4; milk: 1e2-1e3; saliva: 2e2-2e3).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab108799

实验方案

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108799)参考文献

This product has been referenced in:
  • Rosser CJ  et al. Urinary protein biomarker panel for the detection of recurrent bladder cancer. Cancer Epidemiol Biomarkers Prev 23:1340-5 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24714076) »
  • Miyake M  et al. Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection. BMC Urol 13:42 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24011266) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.




ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

Unfortunately, neither of them has cross-reactivity with mouse
Read More

Thank you for contacting us.

You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.


I hope this information is helpful to you. Please do no...

Read More

Thank you for your message.

I can confirm that all three of the Alpha 1 anti trypsin proteins in our catalog are from human, and are full length. Therefore, theoretically, they could be detected by the antibodies in the kit and be used for y...

Read More

Thank you for contacting Abcam and I do apologize for the long delay in providing you with this information.

The standard is a purified protein from human source. The assay should work for cell culture over express alpha1 antitripsin. Both na...

Read More

Thank you for contacting us. The standard provided should only be 100 ng/ml after reconstitution. At that concentration, the recommended curve is a 1:4 dilution providing points: 25, 6.25, 1.56, and 0.391 ng/ml. Concentrations above 100 ng/ml are dange...

Read More

Thank you very much for your reply.

We haven't done any studies to determine the heterogeneity of the detection antibody reagent and I don't know of amethod to really predict that accurately as it's quite complex, but Iassumethere would be ba...

Read More

Thank you very much for your reply.

Since the kits haven't been tested with non-glycosylated forms of alpha 1 antitrypsin, we don't have any data in this regard and can't say for sure. Since the antibodies used in the kit are polyclonal and r...

Read More

Thank you very much for your call earlier this month and for your patience while I have been working with the lab to get this information for you. I sincerely apologize for the extended delay.

The standard is a native protein isolated from hu...

Read More

Thank you for contacting us.

Unfortunately we have not tested this kit with Plasma samples however keeping in mind the recommended sample type, it is more likely that this kit will be suitable for plasma samples also.

As we have not ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"