人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1) (ab108798)

概述

  • 产品名称人alpha 1 Antitrypsin ELISA试剂盒(SERPINA1)
    参阅全部 alpha 1 Antitrypsin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.7%
    批次间
    样品 n Mean SD CV%
    Overall 8.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Saliva, Milk, Serum, Plasma, Cell Lysate
  • 检测类型Competitive
  • 灵敏度
    = 0.018 µg/ml
  • 范围
    0.039 µg/ml - 40 µg/ml
  • 复检

    98 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s alpha 1 Antitrypsin (SERPINA1) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha 1 antitrypsin levels in plasma and serum.


    An alpha 1 antitrypsin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated alpha 1 Antitrypsin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of alpha 1 antitrypsin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab189579) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • 组织特异性Ubiquitous. Expressed in leukocytes and plasma.
  • 疾病相关Alpha-1-antitrypsin deficiency
  • 序列相似性Belongs to the serpin family.
  • 结构域The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • 翻译后修饰N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • 细胞定位Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 别名
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Alpha 1 Antitrypsin measured in biological fluids showing quantity (μg) per mL of tested sample. Serum and plasma samples diluted 100-8100 fold. Human milk and saliva diluted 10 fold. Hu urine diluted 1000 fold.

实验方案

Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) (ab108798)参考文献

This product has been referenced in:
  • Abdullah MI  et al. Tissue and serum samples of patients with papillary thyroid cancer with and without benign background demonstrate different altered expression of proteins. PeerJ 4:e2450 (2016). Competitive ELISA ; Human . Read more (PubMed: 27672505) »

See 1 Publication for this product

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ab108798 is for plasma and serum samples.

ab108799 is for urine, milk, saliva and cell culture samples

Unfortunately, neither of them has cross-reactivity with mouse
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You can use ab108799 for serum, you will just have to optimize the dilutions to get on the standard curve. Our serum samples were at 6000X.


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We unfortunately have not tested these products with sputum samples. Theoretically, these products will be OK however we cannot guarantee as we do not have tested data. The best way for using these products is by i...

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Could you please confirm and explain why the streptavidin conjugate reagent is insufficient for 96 wells plate? Is there any shortage in the volume provided or the reagent does not work properly?...

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