人Alpha 1 acid Glycoprotein ELISA试剂盒(AGP) (ab108854)

概述

  • 产品名称人Alpha 1 acid Glycoprotein ELISA试剂盒(AGP)
    参阅全部 Alpha 1 Acid Glycoprotein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5%
    批次间
    样品 n Mean SD CV%
    Overall 9.7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Saliva, Milk, Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.1 µg/ml
  • 范围
    0.063 µg/ml - 4 µg/ml
  • 复检

    = 98.5 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitive measurement of Alpha 1 acid Glycoprotein in plasma and serum.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Alpha 1 acid Glycoprotein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A1AG1_HUMAN
    • AGP
    • AGP 1
    • AGP A
    • AGP1
    • Alpha 1 acid glycoprotein
    • Alpha-1-acid glycoprotein 1
    • Epididymis secretory sperm binding protein Li 153w
    • HEL S 153w
    • OMD 1
    • ORM
    • ORM1
    • Orosomucoid 1
    • Orosomucoid-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) 图像

  • Alpha1 acid Glycoprotein measured in various biological fluids showing quantity (ng) per mL of sample tested

  • Standard curve : mean of duplicates (+/-SD)

实验方案

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) (ab108854)参考文献

ab108854 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Unfortunately, we do not have any Human serum available in our catalog that could be used as control and the kit ab108854 (http://www.abcam.com/ab108854) comes with Human AGP Standard but not with control serum.
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Thank you for your reply.

We will update the graph, as it indeed confusing. When +2, 1, 0, -1 and -2is written, it means actually 102, 101, 100, 10-1, 10-2 so 100, 10, 1, 0.1 and 0.01.

Please excuse for this error. As said, I alread...

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Thank you for contacting us and your patience while we were investigating this issue.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product tha...

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

Plasma a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"