人Alpha 1 acid Glycoprotein ELISA试剂盒(AGP) (ab108852)

概述

  • 产品名称人Alpha 1 acid Glycoprotein ELISA试剂盒(AGP)
    参阅全部 Alpha 1 Acid Glycoprotein 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 3 ng/ml
  • 范围
    3.125 ng/ml - 200 ng/ml
  • 复检

    = 97 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Alpha 1 acid Glycoprotein (AGP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of AGP levels in cell culture supernatant, saliva, milk, and urine.

    An Alpha 1 acid Glycoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Alpha 1 acid Glycoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Alpha 1 acid Glycoprotein captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • A1AG1_HUMAN
    • AGP
    • AGP 1
    • AGP A
    • AGP1
    • Alpha 1 acid glycoprotein
    • Alpha-1-acid glycoprotein 1
    • Epididymis secretory sperm binding protein Li 153w
    • HEL S 153w
    • OMD 1
    • ORM
    • ORM1
    • Orosomucoid 1
    • Orosomucoid-1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) 图像

  • Representative Standard Curve using ab108852.

实验方案

Human Alpha 1 acid Glycoprotein ELISA Kit (AGP) (ab108852)参考文献

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Thank you for your inquiry.

I can recommend ab108854 for your customer since this kit is tested and guaranteed for plasma and serum samples. ab108852 is not.

Please find the protocol booklet for ab108854 attached.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"