概述

  • 产品名称人Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4%
    批次间
    样品 n Mean SD CV%
    Overall 8.7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    3.125 ng/ml - 200 ng/ml
  • 复检

    96 %

  • 检测时间
    3h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Albumin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性Plasma.
  • 疾病相关Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Albumin ELISA Kit 图像

  • Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).

  • Albumin in HepG2 (diluted 1:50-200) and HeLa (undiluted) supernatants, heparin-treated plasma and serum (5e5-5e6), urine (100-500), milk (5000-30000), saliva (200-2000) was measured in duplicates (+/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Human albumin measured in various samples showing mass (microgram) per mL sample tested

  • Representative Standard Curve using ab108788

实验方案

Human Albumin ELISA Kit (ab108788)参考文献

This product has been referenced in:
  • Tangedal S  et al. Comparison of inflammatory markers in induced and spontaneous sputum in a cohort of COPD patients. Respir Res 15:138 (2014). Human . Read more (PubMed: 25398249) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry. Ab108788 is validated for use with milk samples. The Diluent N does not contain BSA or casein but a BSA like protein. It does not interfere with human albumin measurement or cross react.

The albumin ELISA ab108788 is designed to be able to detect the relatively low amounts (compared to serum and plasma) of albumin in the sample types listed on the datasheet: urine, saliva, milk, cerebrospinal fluid and cell culture supernatants. It wil...

Read More

The laboratory has confirmed that the "10X Diluent Concentrate N" does not contain BSA or casein. It contains other proteins that have no cross-reactivity with human albumin.

Yes, we added this diluent to our catalog: Diluent Concentrate (10x) ab172376.

While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.


The source of the kit ab108788 informed me that they have never tested this kit with horse serum. They have tested it with BSA and they did not see any significant cross-reactivity.

The antibodies used in this kit are optimized for use in kit only. These are not available separately. We have other antibodies available in our catalogue that can be used to develop the assay e.g. ab10241/ ab2406, ab117455

Abreview for Albumin Human ELISA kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2 cells)
Specification: HepG2 cells
Amount of Sample: 0.2ug albumin standard µg
Type of Kit Used: ELISA
Positive control: Provided Albumin Standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human albumin. Did not recognize bovine albumin in FBS.
Username

Abcam user community

Verified customer

提交于 Jul 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"