人Albumin ELISA试剂盒(ab108787)

概述

  • 产品名称人Albumin ELISA试剂盒
    参阅全部 Albumin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.8%
    批次间
    样品 n Mean SD CV%
    Overall 8.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 1.5 µg/ml
  • 范围
    1.563 µg/ml - 100 µg/ml
  • 复检

    97 %

  • 检测时间
    2h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性Plasma.
  • 疾病相关Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human Albumin ELISA Kit 图像

  • Representative Standard Curve using ab108787.

实验方案

Human Albumin ELISA Kit (ab108787)参考文献

ab108787 has not yet been referenced specifically in any publications.

Product Wall

We have used human plasma at a dilution of 10,000x. Rat plasma was also tested at 50x but there was no cross-reactivity.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining consistent results from this kit.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forELISA and h...

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Thank you very much for your call last week and for your patience while I have worked on your enquiry.

The lab has senta vial of the wash buffer, which we are sending to you free of charge on the order ***. This vial should arrive tomorrow. <...

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Thank you very much for your call this week and for your patience while I've been in touch with the lab.

The lab scientists do not recommend using a different buffer with this kit, as the results will be less than optimal. I'm arranging for a...

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DISCOUNT CODE: XXXXXXX
Expiration date: 15 June 2012

I am very pleased to hear you would like to accept our offer and test ab108787 with cell culture supernatant. This code will give you 1 free Kit before the expiration date. To redeem ...

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I am happy in issuing a discount code. Could you send the email and contact address of end user?
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Thank you for your enquiry and interest.
We would be very much interested in these data. Will the customer measure the secreted Albumin into the medium? Would it be possible to get some further information about the experim...

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Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"