概述

  • 产品名称人Adiponectin ELISA试剂盒
    参阅全部 Adiponectin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Urine, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 复检

    < 96 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.59 83% - 107%
    Serum 93.57 83% - 104%
    Plasma 90.29 80% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Adiponectin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Adiponectin coated on a 96-well plate. Standards and samples are pipetted into the wells and Adiponectin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Adiponectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Adiponectin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimisation may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
  • 组织特异性Synthesized exclusively by adipocytes and secreted into plasma.
  • 疾病相关Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
    Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
  • 序列相似性Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • 结构域The C1q domain is commonly called the globular domain.
  • 翻译后修饰Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
    HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
    O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • 30 kDa adipocyte complement related protein
    • 30 kDa adipocyte complement-related protein
    • ACDC
    • ACRP30
    • ADIPO_HUMAN
    • Adipocyte
    • Adipocyte C1q and collagen domain containing protein
    • Adipocyte complement related 30 kDa protein
    • Adipocyte complement related protein of 30 kDa
    • Adipocyte complement-related 30 kDa protein
    • adipocyte-specific secretory protein
    • Adiponectin
    • Adiponectin precursor
    • adiponectin, C1Q and collagen domain containing
    • Adipoq
    • Adipose most abundant gene transcript 1
    • Adipose most abundant gene transcript 1 protein
    • Adipose specific collagen like factor
    • ADIPQTL1
    • ADPN
    • APM 1
    • apM-1
    • APM1
    • C1q and collagen domain-containing protein
    • GBP28
    • Gelatin binding protein
    • Gelatin binding protein 28
    • Gelatin-binding protein
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab99968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Adiponectin ELISA Kit 图像

  • Adiponectin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 2700-24300 fold. Rat and mouse serum were diluted 1-3 fold. Human urine and saliva were diluted 300-2700 fold.

  • Representative Standard Curve using ab99968

  • Representative Standard Curve using ab99968

实验方案

Human Adiponectin ELISA Kit (ab99968)参考文献

This product has been referenced in:
  • Strøm Halden TA  et al. Short-term efficacy and safety of sitagliptin treatment in long-term stable renal recipients with new-onset diabetes after transplantation. Nephrol Dial Transplant 29:926-33 (2014). Read more (PubMed: 24452849) »
  • Schlottmann I  et al. Calcium-dependent release of adipocyte fatty acid binding protein from human adipocytes. Int J Obes (Lond) N/A:N/A (2013). ELISA ; Human . Read more (PubMed: 24352293) »

See all 3 Publications for this product

Product Wall

Human Adiponectin ELISA Kit testing for rhesus macaques

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This kit is suitable for rhesus macaques. Plasma of 17 rhesus macaques were tested and yielded concentrations comparable to human levels and other published studies of rhesus macaques.
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提交于 Nov 02 2015

Thank you for contacting us. I sent your inquiry to the lab, they did not send original data but sent the reply below. Please let me know if you have any more questions.



. A 30,000-fold dilution is quite high but our Human IgG ELISA ...

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Thank you for contacting Abcam.

Unfortunately we have not tested pig samples with theab99968 Human Adiponectin ELISA kit, so we do not know the cross reactivity.Both antibodies used in this kit are mouse monoclonal and the epitope recognized ...

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Thank you for your enquiry.

It is possible that high viscosity of the sample can cause interference with the results. However, I would like to state that we have not tested media with such a high fat content and viscosity, therefore we do no...

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I'm happy to help! Please let me know if you need anything further.

The biotinylated anti-human adiponectin antibody is monoclonal and is raised from mice.

I hope this is helpful. Please contact us again if you have any further questions.

Thank you for your reply and for your patience. It has taken a day or two to confirm this information for you.

ab99968 Adiponectin Human ELISA Kitas been successfully tested with EDTA-plasma, heparin-plasma, and citrate-plasma.

Ot...

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Thank you for your recent telephone enquiry.

I can confirm that the matched pair of antibodies for the Human Adiponectin ELISA kit (ab99968) have an epitope in the globular region (aa 104-244). Therefore, this kit will recognize trimers and ...

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Thank you for contacting us. The capture antibody for the Adiponectin Human ELISA Kits are a monoclonal mouse antibody for kit ab99968 and a polyclonal antibody for kit ab108786 I hope this information is helpful to you. Please do not h...

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