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Abcam’s anti-Adenovirus IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Adenovirus in Human serum and plasma.
A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgA sample captured in plate.
|组件||标识符||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Adenovirus (IgA) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Adenovirus anti-IgA HRP Conjugate||Colored violet; Black cap||1 x 20ml|
|Adenovirus IgA Cut-off Control||Colored yellow; Green cap||1 x 3ml|
|Adenovirus IgA Negative Control||Colored yellow; Blue cap||1 x 2ml|
|Adenovirus IgA Positive Control||Colored yellow; Red cap||1 x 2ml|
|Cover foil||1 unit|
|IgA Sample Diluent||Colored yellow; White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108704 has not yet been referenced specifically in any publications.