人Activated Protein C - DEGR (active site blocked) (ab81574)
概述
- 产品名称人Activated Protein C - DEGR (active site blocked)
参阅全部 Protein C 蛋白酶 - 蛋白长度Full length protein
描述
- 性质Native
- 来源Native
- 氨基酸序列
- 种属Human
相关产品
- Related Products
技术指标
Our Abpromise guarantee covers the use of ab81574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性<1% APC activity. Assay is performed to determine percent contaminating APC activity. APC activity is based on a APC standard curve. Chromagenic assay uses Spectrozyme-TH as substrate.
- 应用
SDS-PAGE
- 纯度> 95
% SDS-PAGE.
- 形式Liquid
- 补充说明
This product is the active site-blocked form containing Dansyl-EGR-chloromethlyketone. ab62396 does not have the active site blocked.
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Concentration information loading...
制备和贮存
- 稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 20mM HEPES, 150mM Sodium chloride, pH 7.4
常规信息
-
别名
- Activation peptide
- Anticoagulant protein C
- APC
see all - 功能Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
- 组织特异性Plasma; synthesized in the liver.
- 疾病相关Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive - 序列相似性Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. - 翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. - 细胞定位Secreted. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
Human Activated Protein C - DEGR (active site blocked) (ab81574)参考文献
ab81574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"