人Activated Protein C - DEGR (active site blocked) (ab81574)
概述
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产品名称人Activated Protein C - DEGR (active site blocked)
参阅全部 Protein C 蛋白酶 -
蛋白长度Full length protein
描述
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性质Native
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来源Native
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氨基酸序列
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种属Human
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab81574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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生物活性<1% APC activity. Assay is performed to determine percent contaminating APC activity. APC activity is based on a APC standard curve. Chromagenic assay uses Spectrozyme-TH as substrate.
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应用
SDS-PAGE
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纯度> 95 % SDS-PAGE.
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形式Liquid
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补充说明
This product is the active site-blocked form containing Dansyl-EGR-chloromethlyketone. ab62396 does not have the active site blocked.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 20mM HEPES, 150mM Sodium chloride, pH 7.4
常规信息
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别名
- Activation peptide
- Anticoagulant protein C
- APC
see all -
功能Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
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组织特异性Plasma; synthesized in the liver.
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疾病相关Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
序列相似性Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
细胞定位Secreted. Golgi apparatus. Endoplasmic reticulum.
- Information by UniProt
Human Activated Protein C - DEGR (active site blocked) (ab81574)参考文献
ab81574 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"