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Our Abpromise guarantee covers the use of ab78467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
ab78467 staining zebrafish eye sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 10%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 21°C, followed by staining with ab78467 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. HuC expression can be observed in amacrine and retinal ganglion cells (in green).