The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Detects bands of approximately 48 & 40 kDa (predicted molecular weight: 50 & 38 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probable serine protease.
Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.
Belongs to the peptidase S1B family. Contains 1 IGFBP N-terminal domain. Contains 1 Kazal-like domain. Contains 1 PDZ (DHR) domain.