概述
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产品名称Anti-htrA1抗体
参阅全部 htrA1 一抗 -
描述兔多克隆抗体to htrA1
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经测试应用适用于: ICC/IF, WB, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human htrA1.
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阳性对照
- In western blot, ab113927 gave a positive signal in the following whole cell lysates: MCF7; JEG3; HEK293; HepG2. In Immunohistochemistry, this antibody gave a positive signal in a formalin-fixed paraffin-embedded human placenta tissue section. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line:HepG2.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab113927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
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组织特异性Expressed in a variety of tissues, with strongest expression in placenta.
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疾病相关Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries. -
序列相似性Belongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain. -
细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 282326 Cow
- Entrez Gene: 5654 Human
- Entrez Gene: 56213 Mouse
- Entrez Gene: 65164 Rat
- Omim: 602194 Human
- SwissProt: F1N152 Cow
- SwissProt: Q92743 Human
- SwissProt: Q9R118 Mouse
see all -
别名
- ARMD7 antibody
- CARASIL antibody
- High-temperature requirement A serine peptidase 1 antibody
see all
Anti-htrA1 antibody 图像
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Western blot - Anti-htrA1 antibody (ab113927)All lanes : Anti-htrA1 antibody (ab113927) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 51 kDa
Observed band size : 51 kDa
Additional bands at : 36 kDa,42 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-htrA1 antibody (ab113927)IHC image of ab113927 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab113927, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times -
Immunocytochemistry/ Immunofluorescence - Anti-htrA1 antibody (ab113927)ICC/IF image of ab113927 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab113927 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) HeLa and MCF-7 cell types at 5ug/ml.
实验方案
Anti-htrA1 antibody (ab113927)参考文献
ab113927 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"