概述

  • 产品名称Anti-HtrA 1 + 2 + 3 + 4抗体
  • 描述
    兔多克隆抗体to HtrA 1 + 2 + 3 + 4
  • 特异性This antibody recognises both Isoform 1 and Isoform 2.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cat
  • 免疫原

    Synthetic peptide:

    IVSTAQREGRELGLR

    , corresponding to amino acids 273-287 of Human HtrA3. This immunogen sequence is 80% homologous in HtrA1 and 71% homologous in HtrA2 and HtrA4

  • 阳性对照
    • Heart lysate. Widely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.2% Gelatin, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明ICC/IF: Use at an assay dependent dilution (PMID 20154083).
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 43 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Anti-HtrA 1 + 2 + 3 + 4 antibody 图像

    • All lanes : Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 3 µg/ml

      Lane 1 : Human heart lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Rat heart lysate
    • Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112) at 2 µg/ml + Rat heart lysate

      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 65 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    • ab22112 at a 1/200 dilution staining HtrA3 in human bronchial cell line BEAS-2B by Immunocytochemistry/ Immunofluorescence.

      Cells were treated with 20µM etoposide for 24 hours.

    Anti-HtrA 1 + 2 + 3 + 4 antibody (ab22112)参考文献

    This product has been referenced in:
    • Beleford D  et al. High temperature requirement A3 (HtrA3) promotes etoposide- and cisplatin-induced cytotoxicity in lung cancer cell lines. J Biol Chem 285:12011-27 (2010). ICC/IF ; Human . Read more (PubMed: 20154083) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab22112.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"