概述

  • 产品名称
    Anti-HTLV I p24抗体[46/3.24.4]
  • 描述
    小鼠单克隆抗体[46/3.24.4] to HTLV I p24
  • 特异性
    Reacts with Human T-Lymphotropic Virus Type I (HTLV I) core p24. Cross reactivity with p24 from HTLV II has been observed.
  • 经测试应用
    适用于: ICC/IF, Radioimmunoprecipitation, WB, ELISAmore details
  • 免疫原

    HTVL viral lysate

  • 常规说明


    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    Purified from serum-free culture supernatant.
  • Primary antibody说明
    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.
  • 克隆
    单克隆
  • 克隆编号
    46/3.24.4
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 19008946
Radioimmunoprecipitation Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    Human T-lymphotropic virus (HTLV) belongs to the family Retroviridae, genus Deltaretrovirus. It is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma in adults and may also be involved in certain demyelinating diseases. Adult T-lymphotropic virus (ATLV) is a strain of this disease that effect primarily adults. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • 细胞定位
    Core protein
  • 别名
    • CA antibody
    • Capsid protein p24 antibody
    • p24 antibody

图片

  • Immunofluorescence analysis of HTLV-1-infected T cells, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde before incubating with 10% normal goat serum and 0.05% saponin for 30 minutes. Cells were incubated with primary antibody and a fluoroscein-conjugated anti-mouse IgG was used as the secondary antibody.
  • Immunofluorescence analysis of HeLa cells transiently transfected with pEYFP-N3-HTLV-1 Gag, staining HTLV I p24 with ab9081.

    Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% normal donkey serum for 30 minutes. Samples were incubated with primary antibody (1/150 in blocking solution) for 2 hours at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/250) was used as the secondary antibody.

文献

This product has been referenced in:
  • Martin-Latil S  et al. Transcytosis of HTLV-1 across a tight human epithelial barrier and infection of subepithelial dendritic cells. Blood 120:572-80 (2012). Read more (PubMed: 22589473) »
  • Grigsby IF  et al. Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry. Retrovirology 7:75 (2010). WB, ICC/IF . Read more (PubMed: 20854688) »

See all 3 Publications for this product

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