The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 10 µg/ml. The Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性Herpes simplex virus (HSV) ICP27 (Infected cell polypeptide 27) is an essential and multifunctional regulator of viral gene expression that modulates RNA splicing, polyadenylation, and nuclear export. ICP27 Belongs to the herpesviruses UL69 family. It is involved in the down-regulation of viral immediate-early genes and acts in combination with ICP0 and ICP4 as an activator of late genes.
ICP47 protein antibody
Immediate early protein IE12 antibody
Infected cell protein 47 antibody
US12 protein antibody
Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] 图像
Western blot - HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631)
All lanes : Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) at 1/1000 dilution
Lane 1 : Mock-infected HeLa cells Lane 2 : HeLa cells infected with wt HSV1 virus (KOS strain, MOI of 2) for 16 hours
Secondary Donkey anti-mouse IgG conjugated to HRP at 1/3000 dilution