Anti-HSV1+ HSV2 ICP27抗体[8.F.137B] (ab31631)

概述

  • 产品名称Anti-HSV1+ HSV2 ICP27抗体[8.F.137B]
    参阅全部 HSV1+ HSV2 ICP27 一抗
  • 描述
    小鼠单克隆抗体[8.F.137B] to HSV1+ HSV2 ICP27
  • 特异性Recognizes ICP27 of HSV I & II. Reactive in IFA of infected cells and immunoprecipitation of specific viral protein.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    Reacts with Herpes Simplex Virus Not yet tested in other species.
  • 免疫原

    Herpes Simplex Regulatory ICP27.

  • 常规说明The Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: None
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号8.F.137B
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 10 µg/ml. The Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Herpes simplex virus (HSV) ICP27 (Infected cell polypeptide 27) is an essential and multifunctional regulator of viral gene expression that modulates RNA splicing, polyadenylation, and nuclear export. ICP27 Belongs to the herpesviruses UL69 family. It is involved in the down-regulation of viral immediate-early genes and acts in combination with ICP0 and ICP4 as an activator of late genes.
    • 别名
      • ICP47 protein antibody
      • Immediate early protein IE12 antibody
      • Infected cell protein 47 antibody
      • US12 antibody
      • US12 protein antibody
      see all

    Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] 图像

    • All lanes : Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) at 1/1000 dilution

      Lane 1 : Mock-infected HeLa cells
      Lane 2 : HeLa cells infected with wt HSV1 virus (KOS strain, MOI of 2) for 16 hours

      Secondary
      Donkey anti-mouse IgG conjugated to HRP at 1/3000 dilution

      This image is courtesy of an anonymous Abreview

      See Abreview

    Anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631)参考文献

    This product has been referenced in:
    • Conwell SE  et al. Identification of TRIM27 as a novel degradation target of herpes simplex virus 1 ICP0. J Virol 89:220-9 (2015). WB . Read more (PubMed: 25320289) »
    • Gholami S  et al. Role of MAPK in oncolytic herpes viral therapy in triple-negative breast cancer. Cancer Gene Ther 21:283-9 (2014). Read more (PubMed: 24924199) »

    See all 11 Publications for this product

    Product Wall

    The immunogen used to raise the anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) was HSV-1(HFEM)tsLB2 infected cell extract. The antibody recognizes ICP27 in HSVI and HSVII. No further epitope mapping has been carried out.

    Thank you for your inquiry. Unfortunately, we do not have any ICP27 protein available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Vero cells)
    Loading amount 10 µg
    Specification Vero cells
    Treatment Infected with HSV1 KOS
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 18 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 10 µg
    Specification HeLa cells
    Treatment infected with HSV1 KOS
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"