Anti-HSV1+ HSV2 ICP27抗体[1-L-11] (ab31631)

概述

  • 产品名称
    Anti-HSV1+ HSV2 ICP27抗体[1-L-11]
    参阅全部 HSV1+ HSV2 ICP27 一抗
  • 描述
    小鼠单克隆抗体[H1113] to HSV1+ HSV2 ICP27
  • 特异性
    Recognizes ICP27 of HSV I & II. Reactive in IFA of infected cells and immunoprecipitation of specific viral protein. The clone number has been updated from (8.F.137B) to (H1113) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    Reacts with Herpes Simplex Virus Not yet tested in other species.
  • 免疫原

    Herpes Simplex Regulatory ICP27.

  • 常规说明

    The Herpes Simplex Virus 1 protein ICP27 contains 512 amino acids and has an apparent molecular weight of 63kDa.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab53480 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: None
    Constituents: 40% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    H1113
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 10 µg/ml.

靶标

  • 相关性
    Herpes simplex virus (HSV) ICP27 (Infected cell polypeptide 27) is an essential and multifunctional regulator of viral gene expression that modulates RNA splicing, polyadenylation, and nuclear export. ICP27 Belongs to the herpesviruses UL69 family. It is involved in the down-regulation of viral immediate-early genes and acts in combination with ICP0 and ICP4 as an activator of late genes.
  • 别名
    • ICP47 protein antibody
    • Immediate early protein IE12 antibody
    • Infected cell protein 47 antibody
    • US12 antibody
    • US12 protein antibody
    see all

图片

  • All lanes : Anti-HSV1+ HSV2 ICP27 antibody [1-L-11] (ab31631) at 1/1000 dilution

    Lane 1 : Mock-infected HeLa cells
    Lane 2 : HeLa cells infected with wt HSV1 virus (KOS strain, MOI of 2) for 16 hours

    Secondary
    Donkey anti-mouse IgG conjugated to HRP at 1/3000 dilution

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

This product has been referenced in:
  • Hu Z  et al. Aqueous synthesized quantum dots interfere with the NF-?B pathway and confer anti-tumor, anti-viral and anti-inflammatory effects. Biomaterials 108:187-96 (2016). Read more (PubMed: 27639114) »
  • Angelova M  et al. Inhibition of O-Linked N-Acetylglucosamine Transferase Reduces Replication of Herpes Simplex Virus and Human Cytomegalovirus. J Virol 89:8474-83 (2015). WB, SDS-PAGE . Read more (PubMed: 26041297) »

See all 13 Publications for this product

客户评价及客户问答

The immunogen used to raise the anti-HSV1+ HSV2 ICP27 antibody [8.F.137B] (ab31631) was HSV-1(HFEM)tsLB2 infected cell extract. The antibody recognizes ICP27 in HSVI and HSVII. No further epitope mapping has been carried out.

Thank you for your inquiry. Unfortunately, we do not have any ICP27 protein available in our catalog. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount
10 µg
Specification
Vero cells
Treatment
Infected with HSV1 KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
infected with HSV1 KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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