概述

  • 产品名称Anti-HSV1抗体
    参阅全部 HSV1 一抗
  • 描述
    兔多克隆抗体to HSV1
  • 特异性This antibody reacts with HSV type I specific antigens and with antigens common to HSV type I and II virus. It reacts with all the major glycoproteins present in the viral envelope as well as with at least one core protein.
  • 经测试应用适用于: IHC - Wholemount, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • 免疫原

    Whole rabbit corneal cells, infected with herpes simplex virus type I (strain Mac Intyre), were solubilized with detergent.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 骨髓瘤unknown
  • 同种型IgG
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC - Wholemount Use at an assay dependent concentration. PubMed: 21998580
IHC-P Use at an assay dependent concentration. This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/500.
ELISA Use at an assay dependent concentration. PubMed: 22003387

靶标

  • 相关性Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.
  • 别名
    • Herpes simplex virus 1 antibody
    • Herpes simplex virus antibody
    • Herpes simplex virus type 1 antibody
    • HSV 1 antibody
    • Human herpesvirus 1 antibody
    • Human herpesvirus type 1 antibody
    see all

Anti-HSV1 antibody 图像

  • ab9533, staining HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae.

    Corneas were fixed in 4% paraformaldehyde, and washed with PBS containing 1% Triton X-100 before being stained with primary antibody. A Dylight®549-conjugated donkey anti-rabbit IgG was used as the secondary antibody.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded HSV1-infected tissue, staining HSV1 with ab9533.

Anti-HSV1 antibody (ab9533)参考文献

This product has been referenced in:
  • Mazzacurati L  et al. Use of miRNA Response Sequences to Block Off-target Replication and Increase the Safety of an Unattenuated, Glioblastoma-targeted Oncolytic HSV. Mol Ther N/A:N/A (2014). Read more (PubMed: 25200130) »
  • McGovern AE  et al. Transneuronal tracing of airways-related sensory circuitry using herpes simplex virus 1, strain H129. Neuroscience 207:148-66 (2012). Read more (PubMed: 22306285) »

See all 7 Publications for this product

Product Wall

I am sorry but the specificity of the antibody has not been studies in detail, I cannot therefore share a great deal of information with you. The antibody has been raised against whole rabbit corneal cells, infected with herpes simplex virus type I. Th...

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Abreviews
Application Flow Cytometry
Sample Saccharomyces cerevisiae Cell (live yeast staining)
Specification live yeast staining
Preparation Cell harvesting/tissue preparation method: harvest cells and wash with 1XPBS
Sample buffer: 1XPBS
Fixation no fixation
Permeabilization No
Gating Strategy HSV-tag expression
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Thank you for your patience in this matter. The product, ab9533 (HSV1 antibody), contains 0.05% sodium azide. Please don't hesitate to contact us again if you have further questions.

It is approx 275 ug/ml

The concentration of this product is 3 mg/ml.

This antibody has not been tested in WB, therefore we can't guarantee results. It will take Abcam approximately 7 days to get the antibody, followed by another 24 hours before it arrives in the US.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"