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Accession number: NM_006512.The HsSAA4 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. SAA4 (Serum amyloid A-4) is expressed in liver but silenced in most other cell types. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most cell types SAA4 is silenced and enriched with histone modifications associated with silencing (e.g. Histone H3 K9 mono methylation, see image with ab9045). Whereas it shows low levels of histone modifications associated with active gene transcription (e.g. Histone H3 K18 acetylation, see image with ab1191).
Our Abpromise guarantee covers the use of ab83610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Real Time PCR||Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.|
ab83610 has not yet been referenced specifically in any publications.
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