HsPGK1 ChIP引物Exon 1 (Forward) (ab83594)

概述

  • 产品名称
    HsPGK1 ChIP引物Exon 1 (Forward)
    参阅全部 PGK1 dna, rna, cdna
  • 常规说明

    Accession number: NM_000291.The HsPGK1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. PGK1 (3-phosphoglycerate kinase 1) is a highly expressed housekeeping gene located on the X chromosome. The primers and probe are located in exon 1, within 1 kb of the transcription start site. In most female cells the locus is enriched with both active (e.g. Histone H3 K18 acetylation, see image with ab1191). and heterochromatic (e.g. Histone H3 K9 tri methylation, see image with ab8898) histone modification marks, as one copy of the gene undergoes X chromosome inactivation. In male cells, this locus is highly enriched with histone modifications associated with active gene transcription whereas it only shows low levels of heterochromatic histone modifications.

  • 经测试应用
    适用于: Real Time PCRmore details

性能

应用

Our Abpromise guarantee covers the use of ab83594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Real Time PCR Use at an assay dependent concentration.

Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.

文献

ab83594 has not yet been referenced specifically in any publications.

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