The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 22.5 kDa).Can be blocked with Human HSPC150 peptide (ab23018).
功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the ubiquitin-conjugating enzyme family.