概述

  • 产品名称Anti-HSPC142抗体
    参阅全部 HSPC142 一抗
  • 描述
    兔多克隆抗体to HSPC142
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 175-225 of Human HSPC142 (NP_054892.2).

  • 阳性对照
    • Hela whole cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab115017 was affinity purified using an epitope specific to HSPC142 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab115017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
    • 序列相似性Belongs to the BABAM1 family.
    • 结构域The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
    • 细胞定位Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BABA1_HUMAN antibody
      • babam1 antibody
      • BRCA1 A complex subunit MERIT40 antibody
      • BRISC and BRCA1-A complex member 1 antibody
      • C19orf62 antibody
      • FLJ20571 antibody
      • HSPC142 antibody
      • hypothetical protein LOC29086 antibody
      • Mediator of Rap80 interactions and targeting 40 kDa antibody
      • Mediator of RAP80 interactions and targeting subunit of 40 kDa antibody
      • MERIT 40 antibody
      • MERIT40 antibody
      • NBA1 antibody
      • New component of the BRCA1 A complex antibody
      • New component of the BRCA1-A complex antibody
      • Uncharacterized protein C19orf62 antibody
      see all

    Anti-HSPC142 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling HSPC142 with ab115017 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of HSPC142 by Western Blot of Immunprecipitate.
      anti-HSPC142, at 1 µg/ml, staining HSPC142 in Hela whole cell lysate immunoprecipitated using ab115017 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-HSPC142 antibody (ab115017)参考文献

    ab115017 has not yet been referenced specifically in any publications.

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