概述

  • 产品名称Anti-HSPC111抗体
    参阅全部 HSPC111 一抗
  • 描述
    小鼠多克隆抗体to HSPC111
  • 特异性Reactive against mammalian transfected lysate
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length Human HSPC111 (AAH40106.1)

  • 阳性对照
    • WB: HeLa, PC-12, Raw 264.7 or NIH/3T3 cell lysate Transfected 293T cell lysate IHC-P: Human esophagus tissue IF/ICC: HeLa cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

Anti-HSPC111 antibody 图像

  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + PC-12 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPC111 antibody (ab88449) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HSPC111 antibody (ab88449) at 1 µg/ml

    Lane 1 : Transfected 293T lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab88449 at 3µg/ml, staining HSPC111 in Human esophagus, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence image staining HSPC111 in HeLa cells. ab88449 was used at 10 ug/ml.

Anti-HSPC111 antibody (ab88449)参考文献

ab88449 has not yet been referenced specifically in any publications.

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