Anti-HSPA4L抗体(ab87241)

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概述

  • 产品名称
    Anti-HSPA4L抗体
    参阅全部 HSPA4L 一抗
  • 描述
    兔多克隆抗体to HSPA4L
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (CHAEHTPEEE IDHTGAKTKS AVSDKQDRLN QTLKKGKVKS IDLPIQSSLC) of human HSPA4L (NP_055093).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • COLO205 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab87241 is purified by a peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • 细胞定位
    Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • 数据库链接
    • 别名
      • 94kDa antibody
      • AI461691 antibody
      • APG 1 antibody
      • Heat shock 70kDa protein 4 like antibody
      • MGC117843 antibody
      • MGC187594 antibody
      • Osmotic stress protein 94 antibody
      • Osp94 antibody
      • OTTMUSP00000027739 antibody
      see all

    图片

    • Western blot - HSPA4L antibody (ab87241)
      Western blot - HSPA4L antibody (ab87241)
      Anti-HSPA4L antibody (ab87241) at 1 µg/ml + COLO205 cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 94 kDa

    实验方案

    文献

    ab87241 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab87241？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    2 reviews or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-HSPA4L antibody

     Below Average
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse breast tumor)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    1e+006 cells
    Specification
    Mouse breast tumor
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    提交于 Mar 15 2017

    Western blot abreview for Anti-HSPA4L antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116 colorectal cancer cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    30 µg
    Treatment
    siRNA (control and HSPA4L)
    Specification
    HCT116 colorectal cancer cell line
    Blocking step
    Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    提交于 Jan 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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