Anti-HSPA4L抗体(ab87241)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to HSPA4L
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 540-589 (CHAEHTPEEE IDHTGAKTKS AVSDKQDRLN QTLKKGKVKS IDLPIQSSLC) of human HSPA4L (NP_055093).

  • 阳性对照
    • COLO205 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab87241 is purified by a peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • 细胞定位
    Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • 数据库链接
  • 别名
    • 94kDa antibody
    • AI461691 antibody
    • APG 1 antibody
    • Heat shock 70kDa protein 4 like antibody
    • MGC117843 antibody
    • MGC187594 antibody
    • Osmotic stress protein 94 antibody
    • Osp94 antibody
    • OTTMUSP00000027739 antibody
    see all

Anti-HSPA4L antibody 图像

  • Anti-HSPA4L antibody (ab87241) at 1 µg/ml + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa

实验方案

Anti-HSPA4L antibody (ab87241)参考文献

ab87241 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse breast tumor)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
1e+006 cells
Specification
Mouse breast tumor
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Mar 15 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colorectal cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Treatment
siRNA (control and HSPA4L)
Specification
HCT116 colorectal cancer cell line
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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