概述

  • 产品名称
  • 描述
    兔多克隆抗体to HSPA4L
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide, corresponding to a region within C terminal amino acids 720-769 (

    RNKDERYDHLDPTEMEKVEKCISDAMSWLNSKMNAQNKLSLTQDPVVKVS

    ) of human HSPA4L (NP_055093).

  • 阳性对照
    • Fetal brain lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified by peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 94 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    The HSPA4L gene, also known as Apg1 or Osp94, belongs to the HSP110 heat shock gene family, which includes three genes encoding highly conserved proteins. HSPA4L is expressed ubiquitously and predominantly in the testis.
  • 细胞定位
    Cytoplasm; Nucleus. Note= May translocate to the nucleus after heat shock
  • 数据库链接
  • 别名
    • 94kDa antibody
    • AI461691 antibody
    • APG 1 antibody
    • Heat shock 70kDa protein 4 like antibody
    • MGC117843 antibody
    • MGC187594 antibody
    • Osmotic stress protein 94 antibody
    • Osp94 antibody
    • OTTMUSP00000027739 antibody
    see all

图片

  • Anti-HSPA4L antibody (ab81221) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

实验方案

文献

This product has been referenced in:
  • Liu XX  et al. Screening targeted testis-specific genes for molecular assessment of aberrant sperm quality. Mol Med Rep 14:1594-600 (2016). Read more (PubMed: 27356588) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cell line)
Loading amount
200000 cells
Specification
Breast cell line
Gel Running Conditions
Reduced Denaturing (12.5 % gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Feb 09 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast cell line)
Total protein in input
3e+006 cells
Specification
breast cell line
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Jan 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (Breast cell line)
Specification
Breast cell line
Fixative
Methanol
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

提交于 Jan 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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