概述

  • 产品名称Anti-HSPA2抗体
    参阅全部 HSPA2 一抗
  • 描述
    小鼠多克隆抗体to HSPA2
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein corresponding to amino acids 1-639 of Human HSPA2 (NP_068814.2).

  • 阳性对照
    • HeLa cell lysate, HSPA2 transfected 293T cell lysate, HeLa cells IHC-P: FFPE human melanoma tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab89130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 70 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70kDa antibody
    • Hcp70.2 antibody
    • Heat shock 70 kDa protein 2 antibody
    • Heat shock protein 2 antibody
    • Heat shock protein 70.2 antibody
    • Heat shock related 70 kDa protein 2 antibody
    • Heat shock-related 70 kDa protein 2 antibody
    • Heat-shock protein, 70-KD, 2 antibody
    • Heat-shock protein, 70-KD, 3 antibody
    • HSP70 2 antibody
    • HSP70 3 antibody
    • Hsp70-2 antibody
    • HSP70-3 antibody
    • HSP70.2 antibody
    • HSP70A2 antibody
    • HSP72 antibody
    • HSP72_HUMAN antibody
    • HSPA2 antibody
    • Hspt70 antibody
    • Hst70 antibody
    • MGC58299 antibody
    • MGC7795 antibody
    • MGC93458 antibody
    • OTTHUMP00000180664 antibody
    • Testis-specific heat shock protein-related antibody
    see all

Anti-HSPA2 antibody 图像

  • IHC image of HSPA2 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab89130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-HSPA2 antibody (ab89130) at 1 µg/ml + HeLa cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HSPA2 antibody (ab89130) at 1 µg/ml

    Lane 1 : HSPA2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ab89130 at 10 µg/ml staining HSPA2 in HeLa cells by Immunofluorescence.

Anti-HSPA2 antibody (ab89130)参考文献

ab89130 has not yet been referenced specifically in any publications.

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