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Synthetic peptide surrounding amino acid 560 of human HSPA2.
Hsp70, one class of stress proteins, is comprised of multiple members, all of which bind ATP in vitro, but that are localized within different intracellular compartments. These include a) the constitutive Hsc70 (or cognate) present within the cytosol/nucleus; b) the stress-inducible Hsp70 present within the cytosol/ nucleus/nucleolus; c) the constitutive glucose regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and d) the glucose regulated 75 kDa protein present within the mitochondria. Hsp70 is typically not expressed in the cell under normal growth conditions, but is expressed at high levels in cells experiencing stress. Consequently, detection of Hsp70 using an antibody specific for the inducible form is useful in ascertaining whether a stress response has occurred in the cell. It should be noted however, that there is a constitutive level of Hsp70 expression in some cell types from primates including human and monkey. Nevertheless increased expression of Hsp70 is observed in such cells following stress.
Our Abpromise guarantee covers the use of ab1428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Can be blocked with Hsp70 peptide (ab55800).|
|ELISA||Use at an assay dependent concentration. PubMed: 17545699|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 17545699
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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