概述

  • 产品名称Anti-HSPA1L抗体
    参阅全部 HSPA1L 一抗
  • 描述
    小鼠单克隆抗体to HSPA1L
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Pig, Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • 免疫原

    Recombinant fragment: KGKISESDKN KILDKCNELL SWLEVNQLAE KDEFDHKRKE LEQMCNPIIT KLYQGGCTGP ACGTGYVPGR PATGPTIEEV D, corresponding to amino acids 561-641 of Human HSPA1L (NP_005518) with a tag of 26 kDa.

  • 阳性对照
    • HeLa and PC12 cell lysates. Recombinant tagged human HSPA1L fragment.

性能

应用

Our Abpromise guarantee covers the use of ab77155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 70 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • 组织特异性Expressed in spermatids.
  • 序列相似性Belongs to the heat shock protein 70 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock 70 kDa protein 1 Hom antibody
    • Heat shock 70 kDa protein 1 like antibody
    • Heat shock 70 kDa protein 1-Hom antibody
    • Heat shock 70 kDa protein 1-like antibody
    • Heat shock 70 kDa protein 1L antibody
    • Heat shock 70kD protein like 1 antibody
    • HS71L_HUMAN antibody
    • HSP70 1L antibody
    • HSP70 HOM antibody
    • HSP70-Hom antibody
    • HSPA1L antibody
    • hum70t antibody
    • Spermatid specific heat shock protein 70 antibody
    see all

Anti-HSPA1L antibody 图像

  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + PC12 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Anti-HSPA1L antibody (ab77155) at 1 µg/ml + Recombinant tagged human HSPA1L fragment at 0.1 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 70 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)

Anti-HSPA1L antibody (ab77155)参考文献

ab77155 has not yet been referenced specifically in any publications.

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