Anti-Hsp90 beta (phospho S254)抗体(ab51136)

概述

  • 产品名称Anti-Hsp90 beta (phospho S254)抗体
    参阅全部 Hsp90 beta 一抗
  • 描述
    兔多克隆抗体to Hsp90 beta (phospho S254)
  • 特异性Detects endogenous levels of HSP90B only when phosphorylated at serine 254.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).

  • 阳性对照
    • Extracts from Hela cells treated with TNF-a, human breast carcinoma tissue. IF/ICC: HeLa cell line

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab51136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 83 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 结构域The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻译后修饰Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta (phospho S254) antibody 图像

  • ICC/IF image of ab51136 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51136, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/500 dilution

    Lane 1 : extracts from Hela cells treated with TNF-a with immunizing peptide
    Lane 2 : extracts from Hela cells treated with TNF-a

    Lysates/proteins at 0.02 µg/ml per lane.


    Predicted band size : 83 kDa
    Observed band size : 88 kDa (why is the actual band size different from the predicted?)
  • Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.Ab51136 at 1/50 dilution staining human breast carcinoma tissue with and without blocking peptide; paraffin embedded.
  • All lanes : Anti-Hsp90 beta (phospho S254) antibody (ab51136) at 1/1000 dilution

    Lane 1 : Mouse heart whole tissue lysate
    Lane 2 : Mouse heart whole tissue lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit polyclonal. at 1/5000 dilution

    Predicted band size : 83 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at room temperature.

    See Abreview

Anti-Hsp90 beta (phospho S254) antibody (ab51136)参考文献

ab51136 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (colon epithelial cell line)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 50 µg
Specification colon epithelial cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Feb 24 2016

Thank you for your inquiry. I can confirm that the immunogen peptide amino acid range for ab51136 is amino acid 245-275 and for ab63562 is amino acid 220-250. I hope this information is helpful and wish you good luck. PS. I send you a quote for...

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