Anti-Hsp90 beta (phospho S226)抗体(ab63562)


  • 产品名称
    Anti-Hsp90 beta (phospho S226)抗体
    参阅全部 Hsp90 beta 一抗
  • 描述
    兔多克隆抗体to Hsp90 beta (phospho S226)
  • 特异性
    Detects Hsp90 beta only when phosphorylated at serine 226.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human Hsp90 beta around the phosphorylation site of serine 226 (E-I-SP-D-D).

  • 阳性对照
    • NIH/3T3 cells.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab63562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.


  • 功能
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性
    Belongs to the heat shock protein 90 family.
  • 结构域
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻译后修饰
    Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    S-nitrosylated; negatively regulates the ATPase activity.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90 alpha family class B member 1 antibody
    • Heat shock protein 90 kDa antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein 90kDa alpha family class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta (phospho S226) antibody 图像

  • All lanes : Anti-Hsp90 beta (phospho S226) antibody (ab63562) at 1/500 dilution

    Lane 1 : extracts from A549 cells, treated with serum (20%, 15mins)
    Lane 2 : extracts from A549 cells, treated with serum (20%, 15mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ab63562 at 1/500 dilution staining NIH/3T3 cells, without (left) and with (right) immunizing peptide.

Anti-Hsp90 beta (phospho S226) antibody (ab63562)参考文献

This product has been referenced in:
  • Wang H  et al. Hsp90a forms a stable complex at the cilium neck for the interaction of signalling molecules in IGF-1 receptor signalling. J Cell Sci 128:100-8 (2015). Read more (PubMed: 25359884) »

See 1 Publication for this product

Product Wall

Western blot
Mouse Cell lysate - whole cell (Bone marrow macrophages)
Gel Running Conditions
Reduced Denaturing (any kD gel (Bio-rad))
Loading amount
1 µg
200 nM human Fc fusion protein
Bone marrow macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Apr 24 2015

Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Human Cell lysate - whole cell (HEK293)
5´M thapsigargin for 3hrs
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Nov 11 2013

Thank you for contacting us. As an isotype control, I would recommend ab37415. For secondary antibodies, you could use any anti-rabbit IgG. If you could let me know which fluorophore you are looking for, I would be happy to suggest a specific product. ...

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Thank you for your inquiry. I can confirm that the immunogen peptide amino acid range for ab51136 is amino acid 245-275 and for ab63562 is amino acid 220-250. I hope this information is helpful and wish you good luck. PS. I send you a quote for...

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