Anti-Hsp90 beta抗体[K3701] (ab82584)

概述

  • 产品名称Anti-Hsp90 beta抗体[K3701]
    参阅全部 Hsp90 beta 一抗
  • 描述
    小鼠单克隆抗体[K3701] to Hsp90 beta
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • 免疫原

    Recombinant human Hsp90 beta protein

  • 阳性对照
    • HeLa, PC12, CHO-K1 cell lysates; Hsp90 native protein; human colon cancer Caco-2 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 6.9
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 克隆单克隆
  • 克隆编号K3701
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Flow Cyt
  • 应用说明Flow Cyt: Use at a concentration of 10 µg/ml.
    WB: 1/1000 (colorimetric). Predicted molecular weight: 83 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
    • 序列相似性Belongs to the heat shock protein 90 family.
    • 结构域The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
    • 翻译后修饰Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
      ISGylated.
      S-nitrosylated; negatively regulates the ATPase activity.
    • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 90 kda heat shock protein beta HSP90 beta antibody
      • D6S182 antibody
      • FLJ26984 antibody
      • Heat shock 84 kDa antibody
      • Heat shock 90kD protein 1, beta antibody
      • Heat shock 90kDa protein 1 beta antibody
      • Heat shock protein 90 alpha family class B member 1 antibody
      • Heat shock protein 90 kDa antibody
      • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
      • Heat shock protein 90kDa alpha family class B member 1 antibody
      • Heat shock protein beta antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-beta antibody
      • HS90B_HUMAN antibody
      • HSP 84 antibody
      • HSP 90 antibody
      • HSP 90 b antibody
      • HSP 90b antibody
      • HSP84 antibody
      • HSP90 BETA antibody
      • hsp90ab1 antibody
      • HSP90B antibody
      • HSPC2 antibody
      • HSPCB antibody
      see all

    Anti-Hsp90 beta antibody [K3701] 图像

    • All lanes : Anti-Hsp90 beta antibody [K3701] (ab82584) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : PC12 cell lysate
      Lane 3 : CHO-K1 cell lysate
      Lane 4 : Hsp90 Native Protein
      Lane 5 : Hsp90 alpha Recombinant Protein (negative control)


      Predicted band size : 83 kDa
      Observed band size : 83 kDa
    • ab82584, at 10 µg/ml, staining p90 beta in human colon cancer Caco-2 cells by flow cytometry (right). Isotype control antibody (left).
    • ab82584 at 1/3000 dilution staining Hsp90 beta in mouse colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining.

    Anti-Hsp90 beta antibody [K3701] (ab82584)参考文献

    ab82584 has not yet been referenced specifically in any publications.

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