Anti-Hsp90 beta抗体(ab80159)


  • 产品名称Anti-Hsp90 beta抗体
    参阅全部 Hsp90 beta 一抗
  • 描述
    兔多克隆抗体to Hsp90 beta
  • 特异性Does not cross react with Hsp90alpha
  • 经测试应用适用于: IHC-P, IHC-Fr, WB, IP, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Purified recombinant human Hsp90 beta

  • 阳性对照
    • HeLa cell lysate (20µg)


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.4
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab80159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
IHC-Fr 1/100.
WB 1/1000. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • 功能Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性Belongs to the heat shock protein 90 family.
  • 结构域The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins.
  • 翻译后修饰Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
    S-nitrosylated; negatively regulates the ATPase activity.
  • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 90 kda heat shock protein beta HSP90 beta antibody
    • D6S182 antibody
    • FLJ26984 antibody
    • Heat shock 84 kDa antibody
    • Heat shock 90kD protein 1, beta antibody
    • Heat shock 90kDa protein 1 beta antibody
    • Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody
    • Heat shock protein beta antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-beta antibody
    • HS90B_HUMAN antibody
    • HSP 84 antibody
    • HSP 90 antibody
    • HSP 90 b antibody
    • HSP 90b antibody
    • HSP84 antibody
    • HSP90 BETA antibody
    • hsp90ab1 antibody
    • HSP90B antibody
    • HSPC2 antibody
    • HSPCB antibody
    see all

Anti-Hsp90 beta antibody 图像

  • ab80159 at 1/100 dilution staining Hsp90 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/10 dilution.

  • All lanes : Anti-Hsp90 beta antibody (ab80159)

    Lane 1 : Molecular weight ladder
    Lane 2 : Human Hsp90 beta protein at 2 µg
    Lane 3 : Human Hsp90 beta protein at 2 µg
    Lane 4 : Molecular weight ladder
    Lane 5 : Human Hsp90 alpha protein
    Lane 6 : Human Hsp90 alpha protein

    Predicted band size : 83 kDa

Anti-Hsp90 beta antibody (ab80159)参考文献

ab80159 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary cancer-associated fibroblasts (prostate))
Loading amount 30 µg
Specification primary cancer-associated fibroblasts (prostate)
Gel Running Conditions Reduced Denaturing (Invitrogen's NuPAGE 4-12%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Alexander Henke

Verified customer

提交于 Aug 02 2011