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Purified full length human Hsp90 beta
Dilute in PBS or medium which is identical to that used in the assay system.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab203126 as a replacement.
Our Abpromise guarantee covers the use of ab58950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa).|
|IP||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.
Cells were grown on sterile coverslips then fixed with 3.7% formaldehyde in PBS for 10 minutes and permeabilized with 0.2% Triton X-100 for 10 minutes at room temperature.
|Flow Cyt||Use 0.01-0.1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.