概述

  • 产品名称
    Anti-Hsp90抗体[EPR3953]
    参阅全部 Hsp90 一抗
  • 描述
    兔单克隆抗体[EPR3953] to Hsp90
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Hsp90.

  • 阳性对照
    • WB: Hsp90 alpha and beta recombinant proteins and HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue and Human kidney tissue IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
    • 序列相似性
      Belongs to the heat shock protein 90 family.
    • 结构域
      The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
    • 翻译后修饰
      ISGylated.
      S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
    • 细胞定位
      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Heat shock 86 kDa antibody
      • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
      • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
      • Heat shock protein HSP 90 alpha antibody
      • Heat shock protein HSP 90 beta antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 84 antibody
      • HSP 86 antibody
      • Hsp 90 antibody
      • HSP86 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90AB1 antibody
      • HSP90B antibody
      • HSPC1 antibody
      • HSPC2 antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    图片

    • All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.

    • ab109248, at 1/100, staining Hsp90 in HeLa cells by Immunofluorescence.

    • ab109248, at 1/100, staining Hsp90 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    • All lanes : Anti-Hsp90 antibody [EPR3953] (ab109248) at 1/1000 dilution

      Lane 1 : Recombinant Human Hsp90 alpha protein (His tag) (ab48801)
      Lane 2 : Recombinant human Hsp90 beta protein (ab80033)

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    文献

    This product has been referenced in:
    • Takata T  et al. Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion. World J Gastroenterol 23:4910-4919 (2017). Read more (PubMed: 28785145) »
    • Guo W  et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . Read more (PubMed: 24465691) »

    See all 3 Publications for this product

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