概述

  • 产品名称
    Anti-Hsp90抗体[AC88]
    参阅全部 Hsp90 一抗
  • 描述
    小鼠单克隆抗体[AC88] to Hsp90
  • 宿主
    Mouse
  • 特异性
    Ab13492 recognises both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa, which are associated with the 30-40S substructures of nuclear hnRNP complexes and share an epitope in common with Hsp90.
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IF, WB, IP, Flow Cyt, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Caenorhabditis elegans, Carp, Fish, Monkey, African green monkey, Rainbow trout
  • 免疫原

    Achlya ambisexualis (water mold) Hsp90.

  • 表位
    The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.
  • 阳性对照
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein. HepG2 cells PC12 cells 3T3 cells Rat2 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is affinity purified.
  • 克隆
    单克隆
  • 克隆编号
    AC88
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 84.7 (alpha) , 83.2 (beta) kDa).
IP Use a concentration of 4 µg/ml. When used in immunoprecipitation, this antibody does not appear to react well with Hsp90 that is bound to steroid receptors or to pp60v-src of Rouse sarcoma virus.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.

靶标

图片

  • Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution + Human fibroblast whole cell lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-mouse polyclonal IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 84.7 (alpha) , 83.2 (beta) kDa


    Exposure time: 20 seconds


    Blocked with 5% Milk for 2 hours at 22°C

    See Abreview

  • Frozen sections of human stomach tissue stained for Hsp90 using ab13492 at 1/100 dilution in immunohistochemical analysis.

  • ab13492 staining Hsp90 (red) and another antibody to C2GnT-M (Golgi enzyme, greeen) in Panc-1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted DyLight® 594-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

     

    See Abreview

  • ab13492 staining Hsp90 in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TRIS-EDTA Buffer. Samples were incubated with primary antibody (1/500) for 30 minutes at 20°C. A HRP-conjugated Goat anti-rabbit/mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab13492 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13492, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab13492 staining Hsp90 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (10 ug/ml).

  • All lanes : Anti-Hsp90 antibody [AC88] (ab13492) at 1/1000 dilution

    Lane 1 : Hsp90 native human protein
    Lane 2 : Hsp90 beta reombinant human protein
    Lane 3 : Hsp90 alpha reombinant human protein
    Lane 4 : Cell lysates prepared from heat shocked Hela cells
    Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
    Lane 6 : Cell lysates prepared from heat shocked PC-12 cells
    Lane 7 : Cell lysates prepared from heat shocked CHO-K1 cells
    Lane 8 : Cell lysates prepared from heat shocked Rat-2 cells

    Predicted band size: 84.7 (alpha) , 83.2 (beta) kDa

  • ICC/IF image of ab13492 stained HepG2 cells (ab7900). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13492, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab13492 Immunoprecipitate OF hSP 90 in human AGS whole cell lysate. 200µg of cell lysate was incubated with primary antibody (1/250 in RIPA buffer) and matrix (Protein A/G) for 16 hours at 4°C.
    For western blotting anti-mouse HRP (ab6728) (1/1000) was used.

    See Abreview

文献

This product has been referenced in:
  • Lv C  et al. The antitumor natural product tanshinone IIA inhibits protein kinase C and acts synergistically with 17-AAG. Cell Death Dis 9:165 (2018). Read more (PubMed: 29416003) »
  • Jeong GR  et al. Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration. Mol Neurodegener 13:8 (2018). Read more (PubMed: 29439717) »

See all 35 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (murine proerythroblast)
Permeabilization
Yes - Tween-20
Specification
murine proerythroblast
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

提交于 Jan 12 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citric Acid
Permeabilization
Yes - Tween-20
Specification
Normal kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 12 2018

Application
Immunocytochemistry
Sample
Human Cultured Cells (Human Renal Epithelial Cells)
Permeabilization
Yes - 0.1% Triton
Specification
Human Renal Epithelial Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Username

Neda Ghousifam

Verified customer

提交于 May 31 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
20 µg
Treatment
Infected with virus
Specification
CHO cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 24 2016

Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Sample
Human Tissue sections (Testis)
Specification
Testis
Permeabilization
Yes - Wash Buffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Mar 31 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (Panc-1)
Specification
Panc-1
Permeabilization
No
Fixative
Paraformaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 19 2013

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Sample
Human Cell lysate - whole cell (Panc-1 cell lysate)
Specification
Panc-1 cell lysate
Treatment
1-control 2- drug KNK437 35 mcm for 1h 3 - drug Brefeldin A 30 mcm for 30 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Cow Cell lysate - whole cell (oviductal epithelial cells)
Specification
oviductal epithelial cells
Blocking step
PVDF Blocking Reagent (TOYOBO) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 06 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Antigen retrieval step
None
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 29 2013

1-10 of 33 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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