Anti-Hsp90抗体[AC-16] (ab6536)

概述

  • 产品名称
    Anti-Hsp90抗体[AC-16]
    参阅全部 Hsp90 一抗
  • 描述
    小鼠单克隆抗体[AC-16] to Hsp90
  • 特异性
    The antibody is reactive with both the constitutive and the inducible forms of HSP90. However, it does not bind to the native form of HSP90. It does not recognize HSP90 from E. coli and yeast.
  • 经测试应用
    适用于: IHC-P, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Human
  • 免疫原

    Full length native protein (purified). (Achlya ambisexualis)

  • 阳性对照
    • HeLa lysate
  • 常规说明
    Monoclonal Anti-Heat Shock Protein 90 (mouse IgG2b isotype) is derived from the AC-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from B1OS mice immunized with heat shock protein 90 purified from the water mold Achlya ambisexualis.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    AC-16
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 83 kDa.

靶标

图片

  • ab6536 at 1/2000 dilution staining Hsp90 in human colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer.

  • ab6536 at 1/2000 dilution staining Hsp90 in mouse colon cancer tissue section by Immunohistochemistry (Bouin's fixed paraffin-embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. A antibody amplifier™ was used for staining. A HRP-conjugated anti mouse secondary was used at 1/10 dilution.

文献

ab6536 has not yet been referenced specifically in any publications.

客户评价及客户问答

ab66536 against hsp90 and ab6535 against hsp70 might be suitable for your purpose. We have posted you the datasheets from the website.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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