概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hsp90
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Drosophila melanogaster, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Hsp90 aa 289-300.
    Sequence:

    ELNKTKPIWTRN

性能

应用

Our Abpromise guarantee covers the use of ab34909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2500. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
ELISA 1/5000 - 1/25000.

靶标

图片

  • All lanes : Anti-Hsp90 antibody (ab34909) at 1/500 dilution

    Lane 1 : Cos7 cells transfected with mutant K294R Hsp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 2 : Cos7 cells transfected with mutant K294R Hsp90, mock treated with DMSO only
    Lane 3 : Cos7 cells transfected wild type Hp90, treated with Trichostatin A (an HDAC inhibitor)
    Lane 4 : Cos7 cells transfected wild type Hp90, mock treated with DMSO only
    Lane 5 : Cos 7 cells transfected with empty vector, treated with Trichostatin A (an HDAC inhibitor)
    Lane 6 : Cos 7 cells transfected with empty vector, mock treated with DMSO only


    Predicted band size : 95 kDa
    Observed band size : 95 kDa

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
1e+007 cells
Gel Running Conditions
Reduced Denaturing (4-20% gradient gels)
Sample
Candida albicans Cell lysate - whole cell (cells grown in liquid YPD)
Specification
cells grown in liquid YPD
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Tissue sections (Eye imaginal disc)
Specification
Eye imaginal disc
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS 0.1% triton
Username

Abcam user community

Verified customer

提交于 Oct 13 2010

Application
Western blot
Sample
Mouse Cell lysate - whole cell (n2a cell)
Loading amount
20 µg
Specification
n2a cell
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 27 2008

Thank you for your interesting enquiry. I have looked at the details for all the antibodies we have raised against Hsp90. There is another one available, rabbit polyclonal ab13494 that will be more suitable for your application. It has been tested in E...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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