概述

  • 产品名称
  • 描述
    兔多克隆抗体to Hsp90
  • 特异性
    Detects 90kD proteins corresponding to the molecular mass of hsp90aß.
  • 经测试应用
    适用于: ELISA, IP, IHC-P, IHC-Fr, ICC/IF, ICC, Flow Cyt, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Human Hsp90.

  • 阳性对照
    • HeLa Cell Lysate (Heat Shocked). Hsp90 Protein.

性能

应用

Our Abpromise guarantee covers the use of ab13495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt 1/250.

(see Abreview).

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/20000 - 1/40000. Detects a band of approximately 90 kDa (predicted molecular weight: 83.2 (beta) , 84.5 (alpha) kDa).

靶标

  • 功能
    Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
  • 序列相似性
    Belongs to the heat shock protein 90 family.
  • 结构域
    The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
  • 翻译后修饰
    ISGylated.
    S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
  • 细胞定位
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Heat shock 86 kDa antibody
    • Heat shock protein 90kDa alpha cytosolic class A member 1 antibody
    • Heat shock protein 90kDa alpha cytosolic class B member 1 antibody
    • Heat shock protein HSP 90 alpha antibody
    • Heat shock protein HSP 90 beta antibody
    • Heat shock protein HSP 90-alpha antibody
    • HS90A_HUMAN antibody
    • HSP 84 antibody
    • HSP 86 antibody
    • Hsp 90 antibody
    • HSP86 antibody
    • HSP90A antibody
    • HSP90AA1 antibody
    • HSP90AB1 antibody
    • HSP90B antibody
    • HSPC1 antibody
    • HSPC2 antibody
    • HSPCAL1 antibody
    • HSPCAL4 antibody
    • Renal carcinoma antigen NY-REN-38 antibody
    see all

Anti-Hsp90 antibody 图像

  • All lanes : Anti-Hsp90 antibody (ab13495) at 1/10000 dilution (2 hours at room temperature)

    Lane 1 : A431 with BSA for 30 minutes at room temperature
    Lane 2 : A549 with BSA for 30 minutes at room temperature
    Lane 3 : HCT116 with BSA for 30 minutes at room temperature
    Lane 4 : Hela with BSA for 30 minutes at room temperature
    Lane 5 : HEK293 with BSA for 30 minutes at room temperature
    Lane 6 : HepG2 with BSA for 30 minutes at room temperature
    Lane 7 : HL-60 with BSA for 30 minutes at room temperature
    Lane 8 : HUVEC with BSA for 30 minutes at room temperature
    Lane 9 : Jurkat with BSA for 30 minutes at room temperature
    Lane 10 : MCF7 with BSA for 30 minutes at room temperature
    Lane 11 : PC3 with BSA for 30 minutes at room temperature
    Lane 12 : T98G with BSA for 30 minutes at room temperature

    Lysates/proteins at 2 µg per lane.
    Blocking peptides at 1.5 % per lane.

    Secondary
    HRP Donkey anti-rabbit IgG, 1 hour at room temperature

    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
  • ab13495 staining Hsp90 in murine RAW 264 (ab7187).7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 4°C and then incubated with ab13495 at a 1/150 dilution. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/500 dilution.

    See Abreview

  • ab13495 staining Hsp90 in Mouse backskin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's fixative solution. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A FITC-conjugated Goat anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

  • ab13495 at a 1/100 dilution staining Hsp90 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. PFA fixed. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor 568 (ab175471).Left image: DAPI staining nuclei (blue)Middle image: Hsp90 (red)Right image: Overlay

    See Abreview

  • ab13495 staining Hsp90 in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    P : Permeabilized; US : Unstained, Red Peak; IgG RB : IgG Rabbit (Isotype Control) ab171870), Blue Peak; HSP90, Green peak.

    See Abreview

  • Anti-Hsp90 antibody (ab13495) at 1/1000 dilution + whole cell lysate prepared from human platelets treated with A23187 for 1 hour at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 83.2 (beta) , 84.5 (alpha) kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    See Abreview

Anti-Hsp90 antibody (ab13495)参考文献

This product has been referenced in:
  • Shephard F  et al. Analysis of Mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion 29:45-52 (2016). WB . Read more (PubMed: 27181046) »
  • Sin YY  et al. Small heat shock protein 20 (Hsp20) facilitates nuclear import of protein kinase D 1 (PKD1) during cardiac hypertrophy. Cell Commun Signal 13:16 (2015). Read more (PubMed: 25889640) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 11 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% Gel)
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 04 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Rat Tissue lysate - whole (rat brain celles)
Specification
rat brain celles
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Oct 10 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 10 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (Glioblastoma cell lines)
Specification
Glioblastoma cell lines
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. Achilleas Mitrakas

Verified customer

提交于 Apr 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (U87MG Human brain glioblastoma)
Specification
U87MG Human brain glioblastoma
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

提交于 Nov 18 2013

Thank you for providing those details.

The sequences provided are only truncations of the proteins and I have therefore not been able to compare them to the immunogens used to raise the antibodies ab2787 and ab13495. I cannot therefore ascert...

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Thank you very much for providing those sequences.

In order to help the customer, could you please provide me the sequence in text format (i.e. in the body of the email, or as a word document). This will allow me to copy and paste the sequenc...

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Thank you for contacting us.

I have been unable to find a sequence for Hsp90 and Hsp70 in bufo bankorensis, I am therefore not able to say if the antibodies are likely to be able to detect the proteins from these samples. Would you be able to...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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