The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 83 kDa). (Colorimetric).
Use at an assay dependent dilution.
功能Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
序列相似性Belongs to the heat shock protein 90 family.
结构域The TPR repeat-binding motif mediates interaction with TPR repeat-containing proteins like the co-chaperone STUB1.
翻译后修饰ISGylated. S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.
细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.