概述

  • 产品名称Anti-Hsp90 alpha抗体[EPR3953]
    参阅全部 Hsp90 alpha 一抗
  • 描述
    兔单克隆抗体[EPR3953] to Hsp90 alpha
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to region within Human Hsp90 alpha.

  • 阳性对照
    • WB: HeLa and 293T cell lysates IHC-P: Human gastric adenocarcinoma tissue and Human kidney tissue IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 85 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Molecular chaperone. Has ATPase activity.
    • 序列相似性Belongs to the heat shock protein 90 family.
    • 翻译后修饰ISGylated.
    • 细胞定位Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EL52 antibody
      • epididymis luminal secretory protein 52 antibody
      • Heat shock 86 kDa antibody
      • heat shock 90kD protein 1, alpha antibody
      • Heat shock 90kD protein 1, alpha like 4 antibody
      • heat shock 90kD protein, alpha-like 4 antibody
      • Heat shock 90kDa protein 1 alpha antibody
      • Heat shock protein 90kDa alpha (cytosolic) class A member 1 antibody
      • Heat shock protein HSP 90-alpha antibody
      • HS90A_HUMAN antibody
      • HSP 86 antibody
      • HSP86 antibody
      • Hsp89 antibody
      • HSP89A antibody
      • Hsp90 antibody
      • HSP90A antibody
      • HSP90AA1 antibody
      • HSP90ALPHA antibody
      • HSP90N antibody
      • HSPC1 antibody
      • HSPCA antibody
      • HSPCAL1 antibody
      • HSPCAL4 antibody
      • HSPN antibody
      • LAP 2 antibody
      • LAP2 antibody
      • lipopolysaccharide-associated protein 2 antibody
      • LPS-associated protein 2 antibody
      • Renal carcinoma antigen NY-REN-38 antibody
      see all

    Anti-Hsp90 alpha antibody [EPR3953] 图像

    • All lanes : Anti-Hsp90 alpha antibody [EPR3953] (ab109248) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 85 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • ab109248, at 1/100, staining Hsp90 alpha in HeLa cells by Immunofluorescence.

    Anti-Hsp90 alpha antibody [EPR3953] (ab109248)参考文献

    This product has been referenced in:
    • Guo W  et al. Targeting GRP75 improves HSP90 inhibitor efficacy by enhancing p53-mediated apoptosis in hepatocellular carcinoma. PLoS One 9:e85766 (2014). IHC ; Human . Read more (PubMed: 24465691) »
    • Guarani V  et al. TIMMDC1/C3orf1 functions as a membrane-embedded mitochondrial complex I assembly factor through association with the MCIA complex. Mol Cell Biol 34:847-61 (2014). Read more (PubMed: 24344204) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"