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Anti-Hsp90 alpha antibody [D7a] 图像
Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 alpha antibody [D7a] (ab59459)This image is courtesy of an Abreview submitted by James Harris
ab59459 staining Hsp90 alpha in the THP-1 monocyte/macrophage cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with PFA, permeabilized with Triton X-100 0.1% and blocked with 5% Serum for 60 minutes at 20°C. Samples were incubated with primary antibody (1/200 1% BSA/5% Goat Serum) for 1 hour at 20°C. An Alexa Fluor®647-conjugated Goat anti-mouse IgG polyclonal(1/500) was used as the secondary antibody.
ab59459 staining Hsp90 alpha in human colon cancer tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave in citrate buffer. The primary antibody was used at dilution of 1/100,000 dilution using antibody amplifier™ system.
Overlay histogram showing HeLa cells stained with ab59459 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59459, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-Hsp90 alpha antibody [D7a] (ab59459)参考文献
This product has been referenced in:
Perez-Branguli F et al. Reverse Signaling by Semaphorin-6A Regulates Cellular Aggregation and Neuronal Morphology. PLoS One11:e0158686 (2016).
Read more (PubMed: 27392094) »
Morlé A et al. Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion. Cell Death Dis6:e1633 (2015).
Read more (PubMed: 25675293) »